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anti cd73 pe  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti cd73 pe
    Anti Cd73 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd73+pe/CD73+Antibody%2C+anti-human%2C+REAfinity/10__20517_slash_evcna__2025__130-51-3-5
    Average 95 stars, based on 57 article reviews
    anti cd73 pe - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles.
    Article Snippet: ASCs at passage 1 were analyzed by flow cytometry with a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 30,000 events. .. Cells were stained for 30 min at 4 C with the following antibodies: anti-CD90-FITC (REA897, Miltenyi Biotec, Bergisch Gladbach, Germany), CD73-PE (REA804, Miltenyi), CD105-PerCP-Cy5.5 (43A3, BioLegend, San Diego, CA, USA) and CD45-PE-Vio770 (REA747, Miltenyi) and analyzed after one wash with FACS-buffer. ..

    Article Title: Comparative analysis of adipose-, bone marrow-, and amniotic membrane-derived MSC secretomes and EVs reveals shared and source-specific therapeutic signatures for osteoarthritis
    Article Snippet: .. A total of 100,000 cells per condition were either left unstained or stained with fluorochrome-conjugated antibodies as follows: anti-CD45-PE-Vio770 (clone REA747), CD73-PE (clone REA804), and CD90-FITC (clone REA897) from Miltenyi Biotec (Bergisch Gladbach, Germany); and CD31-APC (clone WM59), CD105-PerCP/Cy5.5 (clone 43A3), and CD146-APC/Fire750 (clone P1H12) from BioLegend (San Diego, CA, USA). ..

    Article Title: Donor Sites and Harvesting Techniques Affect miRNA Cargos of Extracellular Vesicles Released by Human Adipose-Derived Mesenchymal Stromal Cells.
    Article Snippet: .. ASCs from an abdomen in suspension at Passage 3 were stained for 30 min at 4 ◦C in the dark with the following antibodies and dilutions: 1:20 anti-CD44-APC (REA690, Miltenyi Biotec, Bergisch Gladbach, Germany), 1:50 CD73-PE (REA804, Miltenyi), 1:50 CD90-FITC (REA897, Miltenyi), and 1:50 CD45-PE-Vio770 (REA747, Miltenyi), according to the manufacturer’s instructions. .. After the cell wash with FACS buffer (phosphate buffer, 5% FBS, 0.1% sodium azide), cells were detected by flow cytometry using a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 20,000 events.

    Article Title: Donor Sites and Harvesting Techniques Affect miRNA Cargos of Extracellular Vesicles Released by Human Adipose-Derived Mesenchymal Stromal Cells
    Article Snippet: .. ASCs from an abdomen in suspension at Passage 3 were stained for 30 min at 4 °C in the dark with the following antibodies and dilutions: 1:20 anti-CD44-APC (REA690, Miltenyi Biotec, Bergisch Gladbach, Germany), 1:50 CD73-PE (REA804, Miltenyi), 1:50 CD90-FITC (REA897, Miltenyi), and 1:50 CD45-PE-Vio770 (REA747, Miltenyi), according to the manufacturer’s instructions. .. After the cell wash with FACS buffer (phosphate buffer, 5% FBS, 0.1% sodium azide), cells were detected by flow cytometry using a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 20,000 events.

    Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles
    Article Snippet: ASCs at passage 1 were analyzed by flow cytometry with a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 30,000 events. .. Cells were stained for 30 min at 4 °C with the following antibodies: anti-CD90-FITC (REA897, Miltenyi Biotec, Bergisch Gladbach, Germany), CD73-PE (REA804, Miltenyi), CD105-PerCP-Cy5.5 (43A3, BioLegend, San Diego, CA, USA) and CD45-PE-Vio770 (REA747, Miltenyi) and analyzed after one wash with FACS-buffer. ..

    Article Title: Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications
    Article Snippet: .. ASCs at 90 % confluence in the different media were detached and 100,000 cells were left unstained or stained with the following antibodies: anti-CD45-PE Vio770 clone REA747, CD73-PE clone REA804, CD90-FITC clone REA897 (Miltenyi Biotec, Bergisch Gladbach, Germany) and CD31-APC clone WM59, CD105-PerCP/Cy5.5 clone 43A3 and CD146-APC/Fire750 clone P1H12 (Biolegend, San Diego, CA, USA) in FACS buffer (1 x PBS, 2 % FBS, 1 mM EDTA) following manufacturer's protocol for Abs dilution. ..

    FACS:

    Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles.
    Article Snippet: ASCs at passage 1 were analyzed by flow cytometry with a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 30,000 events. .. Cells were stained for 30 min at 4 C with the following antibodies: anti-CD90-FITC (REA897, Miltenyi Biotec, Bergisch Gladbach, Germany), CD73-PE (REA804, Miltenyi), CD105-PerCP-Cy5.5 (43A3, BioLegend, San Diego, CA, USA) and CD45-PE-Vio770 (REA747, Miltenyi) and analyzed after one wash with FACS-buffer. ..

    Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles
    Article Snippet: ASCs at passage 1 were analyzed by flow cytometry with a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 30,000 events. .. Cells were stained for 30 min at 4 °C with the following antibodies: anti-CD90-FITC (REA897, Miltenyi Biotec, Bergisch Gladbach, Germany), CD73-PE (REA804, Miltenyi), CD105-PerCP-Cy5.5 (43A3, BioLegend, San Diego, CA, USA) and CD45-PE-Vio770 (REA747, Miltenyi) and analyzed after one wash with FACS-buffer. ..

    Article Title: Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications
    Article Snippet: .. ASCs at 90 % confluence in the different media were detached and 100,000 cells were left unstained or stained with the following antibodies: anti-CD45-PE Vio770 clone REA747, CD73-PE clone REA804, CD90-FITC clone REA897 (Miltenyi Biotec, Bergisch Gladbach, Germany) and CD31-APC clone WM59, CD105-PerCP/Cy5.5 clone 43A3 and CD146-APC/Fire750 clone P1H12 (Biolegend, San Diego, CA, USA) in FACS buffer (1 x PBS, 2 % FBS, 1 mM EDTA) following manufacturer's protocol for Abs dilution. ..

    Suspension:

    Article Title: Donor Sites and Harvesting Techniques Affect miRNA Cargos of Extracellular Vesicles Released by Human Adipose-Derived Mesenchymal Stromal Cells.
    Article Snippet: .. ASCs from an abdomen in suspension at Passage 3 were stained for 30 min at 4 ◦C in the dark with the following antibodies and dilutions: 1:20 anti-CD44-APC (REA690, Miltenyi Biotec, Bergisch Gladbach, Germany), 1:50 CD73-PE (REA804, Miltenyi), 1:50 CD90-FITC (REA897, Miltenyi), and 1:50 CD45-PE-Vio770 (REA747, Miltenyi), according to the manufacturer’s instructions. .. After the cell wash with FACS buffer (phosphate buffer, 5% FBS, 0.1% sodium azide), cells were detected by flow cytometry using a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 20,000 events.

    Article Title: Donor Sites and Harvesting Techniques Affect miRNA Cargos of Extracellular Vesicles Released by Human Adipose-Derived Mesenchymal Stromal Cells
    Article Snippet: .. ASCs from an abdomen in suspension at Passage 3 were stained for 30 min at 4 °C in the dark with the following antibodies and dilutions: 1:20 anti-CD44-APC (REA690, Miltenyi Biotec, Bergisch Gladbach, Germany), 1:50 CD73-PE (REA804, Miltenyi), 1:50 CD90-FITC (REA897, Miltenyi), and 1:50 CD45-PE-Vio770 (REA747, Miltenyi), according to the manufacturer’s instructions. .. After the cell wash with FACS buffer (phosphate buffer, 5% FBS, 0.1% sodium azide), cells were detected by flow cytometry using a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA), collecting at least 20,000 events.

    other:

    Article Title: Inflammatory Modulation of Toll-like Receptors in Periodontal Ligament Stem Cells: Implications for Periodontal Therapy
    Article Snippet: CD73 PE , Miltenyi Biotec, Bergisch Gladbach, Germany , 130-112-060.



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